An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures
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چکیده
منابع مشابه
An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures.
X and Y chromosome paints were developed from sorted yak chromosomes for sexing cattle spermatozoa. Clear hybridization signals were obtained for every spermatozoon using a modified sperm decondensation protocol and fluorescence in situ hybridization (FISH). The procedure was evaluated using the established Beltsville sperm sexing technology, which separates spermatozoa by flow cytometry into X...
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متن کاملeffect of combined density gradient centrifugation on x- and y- sperm separation and chromatin integrity
background: it has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. these methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques. objective: this study compared simple density gradient methods and a combin...
متن کاملEffect of combined density gradient centrifugation on X- and Y- sperm separation and chromatin integrity
BACKGROUND It has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. These methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques. OBJECTIVE This study compared simple density gradient methods and a combin...
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ژورنال
عنوان ژورنال: Reproduction
سال: 2001
ISSN: 1470-1626,1741-7899
DOI: 10.1530/reprod/121.4.541